flim spc 150 tcspc module (Becker & Hickl)
90
Structured Review
Becker & Hickl
flim spc 150 tcspc module
Flim Spc 150 Tcspc Module, supplied by Becker & Hickl, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flim+module/tcspc+module/pmc11591579-70-16-21
Average 90 stars, based on 1 article reviews
Flim Spc 150 Tcspc Module, supplied by Becker & Hickl, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flim+module/tcspc+module/pmc11591579-70-16-21
Average 90 stars, based on 1 article reviews
flim spc 150 tcspc module - by Bioz Stars,
2026-09
90/100 stars
Images
1) Product Images from "Unraveling Microviscosity Changes Induced in Cancer Cells by Photodynamic Therapy with Targeted Genetically Encoded Photosensitizer"
Article Title: Unraveling Microviscosity Changes Induced in Cancer Cells by Photodynamic Therapy with Targeted Genetically Encoded Photosensitizer
Journal: Biomedicines
doi: 10.3390/biomedicines12112550
Figure Legend Snippet: Plasma membrane viscosity in HeLa Kyoto cells with KillerRed during PDT. ( A ) Representative FLIM images of cells with both localizations of KillerRed. The bar is 40 µm, applicable to all images. ( B ) Quantification of viscosity of plasma membranes in HeLa Kyoto cells. Means ± SD, n = 100 cells for each time point. * p < 0.05 with control; # p < 0.05 with KillerRed-H2B. CNT KR: control with different localization of KillerRed. H2B: cells with nuclear localization of KillerRed. PM: cells with membrane localization of KillerRed.
Techniques Used: Membrane, Viscosity, Control
Figure Legend Snippet: Plasma membrane microviscosity in HeLa tumor spheroids after PDT with KillerRed localized in the nuclei (H2B) or within the plasma membrane (PM). ( A ) Schematic representation of the spheroid area (shown by the yellow square) imaged by FLIM. The spheroid had adhered to the glass bottom, and the images were acquired from a depth of ~30 μm. Higher-magnification image of the molecular rotor distribution in spheroid cell membranes indicated by the red squares. The scale bar is 80 μm. ( B ) FLIM images and live/dead (LD) assay of control and treated cells in spheroids. Bar = 80 μm. ( C ) Morphology of control and treated spheroids. The scale bar is 80 μm. ( D ) Quantification of membrane microviscosity of spheroid cells after PDT. Means ± SD, n = 4 spheroids, 60 cells in each. ( E ) Quantitative analysis of dead cells in control and treated cell populations, %. * p < 0.05 with control; # p < 0.05 with KillerRed-H2B. CNT KR: control with different localization of KillerRed. H2B: cells with nuclear localization of KillerRed. PM: cells with membrane localization of KillerRed.
Techniques Used: Membrane, Control
Related Articles
Fluorescence:Article Title: Multimodal Nonlinear Microscopy for Therapy Monitoring of Cold Atmospheric Plasma Treatment Article Snippet: The laser passes a long pass dichroic mirror (see e number 3, long pass 600 nm, Zeiss, Germany) before being focused onto the specimen by a 20× microscope objective (see e number 5, Plan-Apochromat, NA 0.8, Zeiss, Germany). .. The fluorescence signal is collected by the microscope objective and filtered from the laser light by the dichroic mirror (see e number 3) and two filters (short pass 650 nm, bandpass 458/64 nm, Semrock, USA) before detection by the Article Title: Multimodal label-free imaging of living dermal equivalents including dermal papilla cells Article Snippet: .. The two-photon excited fluorescence intensity and FLIM images were obtained using a multiphoton tomography MPTflex (JenLab GmbH, Germany) equipped with a tunable 80 MHz, 200 fs Ti:Sa laser MaiTai, and a Article Title: Genetically encoded far-red fluorescent sensors for caspase-3 activity. Article Snippet: .. Fluorescence lifetime imaging (FLIM) FLIM was performed on a laser scanning confocal microscope (LSM 710; Carl Zeiss, Jena, Germany) with a Microscopy:Article Title: Multimodal Nonlinear Microscopy for Therapy Monitoring of Cold Atmospheric Plasma Treatment Article Snippet: The laser passes a long pass dichroic mirror (see e number 3, long pass 600 nm, Zeiss, Germany) before being focused onto the specimen by a 20× microscope objective (see e number 5, Plan-Apochromat, NA 0.8, Zeiss, Germany). .. The fluorescence signal is collected by the microscope objective and filtered from the laser light by the dichroic mirror (see e number 3) and two filters (short pass 650 nm, bandpass 458/64 nm, Semrock, USA) before detection by the Article Title: Genetically encoded far-red fluorescent sensors for caspase-3 activity. Article Snippet: .. Fluorescence lifetime imaging (FLIM) FLIM was performed on a laser scanning confocal microscope (LSM 710; Carl Zeiss, Jena, Germany) with a Tomography:Article Title: Multimodal label-free imaging of living dermal equivalents including dermal papilla cells Article Snippet: .. The two-photon excited fluorescence intensity and FLIM images were obtained using a multiphoton tomography MPTflex (JenLab GmbH, Germany) equipped with a tunable 80 MHz, 200 fs Ti:Sa laser MaiTai, and a Imaging:Article Title: Genetically encoded far-red fluorescent sensors for caspase-3 activity. Article Snippet: .. Fluorescence lifetime imaging (FLIM) FLIM was performed on a laser scanning confocal microscope (LSM 710; Carl Zeiss, Jena, Germany) with a |